EXPERIMENT 5 : TOTAL PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY FROM PLANT MATERIALS 5.1 OBJECTIVE Average: 0. Your rating: Your rating: None. This assay is responsive to any reducing substance, so if applied to other types of samples, large errors could be encountered.

No votes yet. Ainsworth, E., Gillespie, K. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent. 3.2. Gallic acid was used as a reference standard for plotting calibration curve. It has a relative mass response of 0.68, so a wine with 30 mg/L ascorbate must be corrected by subtracting 20.4 mg/L from the total phenol value. The appropriate amount of fi ltered methanol extracts were oxidized with Folin-Ciocalteu reagents and a er 5 minutes was the reaction neutralized with saturated sodium carbonate. Two different colorimetric methods were used over three years and comparative student results indicate that a ferrous ammonium sulfate (FAS) indicator procedure was a good choice for our GOB laboratory. Please show me the step-by-step calculation of total phenolic content determination. The total phenolic content of the extract was determined by the Folin–Ciocalteu method . The total flavonoid concentrations varied from 18.72 to 54.77 mg/g, expressed as rutin equivalents. Determination of total phenol content Total phenolic content was estimated by Folin Ciocalteu’s method. The total phenolic content of Gallic acid samples can be determined spectrophotometrically using the Folin-Ciocalteu reagent (Povichit et al., 2010) and each sample should be done in triplicate. Values in percentage ranged from 27.26 to 89.78%. The total phenolic content was calculated with the help of the graph shown in Figure 1, and the standard curve equation was y = 0.007x + 0.186, where R 2 = 0.992. The determination of total phenolics in foods and fruit juices was used successfully as a laboratory experiment in our undergraduate general, organic, and biological (GOB) chemistry course. 10. ... A demo and practice experiment - Duration: ... 11:31. Total Phenolics by Folin Ciocalteu - Jaime Miranda - Duration: 10:00. Determination of Antioxidant Capacity, Total Phenolic Content and Mineral Composition of Different Fruit Tissue of Five Apple Cultivars Grown in Chile. Given the following: weight of sample = 0.1 g; Absorbance at 760 nm = 0.342 A and Azeez L Total antioxidant activity, phenolic, flavonoid and ascorbic acid contents of Nigerian vegetables., 2011; 2(2) 022-029,African Journal of Food Science and Technology. It has been widely applied in determination of the total phenol/polyphenol content of plant‐derived food and biological samples. 1 ml of aliquots and standard gallic acid (10, 20, 40, 60, 80, 100 µg/ml) was positioned into the test tubes and 5 ml of distilled water and 0.5 ml of Folin Ciocalteu’s reagent was mixed and shaken. The total phenolic content ranged from 27.26 to 89.78 mg/g of dry weight of extract, expressed as gallic acid equivalents. The Folin–Ciocalteu (F‐C) reaction is an antioxidant assay based on electron transfer, which measures the reductive capacity of an antioxidant. The total phenolic content of all ten mushrooms ranged from 0.015-0.075 mg GAE/g dried extract and the total flavonoid content ranged from 0.025-0.131 mg QE/g dried extract. The experimental samples were taken from the top layer of soil after 36 (experiment I) and 35 (experiment II) years following the establishment of the experiments. Total phenolic content. Briefly, 200 μL of crude extract (1 mg/mL) were made up to 3 mL with distilled water, mixed thoroughly with 0.5 mL of Folin–Ciocalteu reagent for 3 min, followed by the addition of 2 mL of 20% (w/v) sodium carbonate. Determination of total flavonoid content. Nat Protoc 2, 875–877 (2007). The total phenolic contents were calculated using the following linear equation based on the calibration curve of gallic acid; A=0.008X+0.0727, R 2 = 0.9967 Tweet. The total phenolic contents (Gallic acid equivalents, mg/g) in the ethanolic and aqueous extracts were … The FRAP values lies between 0.859-2.929 μM TE/g dried extract and the percentage of inhibition observed from DPPH assay ranged from 2%-57% respectively. The total phenolic content expressed in terms of GAE and yield (%) of flower extract was found to be (19.31±1.79) mg of GA/g and 8.69% (w/w) respectively. Evaluation of total phenolic content The total phenolic content was estimated using the modifi ed Folin-Ciocalteu photometric method (You et al., 2007). The total phenolic content of the M. oleifera flower extract was determined by using Folin-Ciocalteu reagent following a slightly modified method of Ainsworth.

Las manzanas (Malus domestica Borkh.) Roya K, Fatemeh G Screening of total phenol and flavonoid content, antioxidant and antibacterial