Steps Carefully pour the bleach into the spray bottle or jar first, then add the water. Wearing the gloves, carefully measure the bleach and add it to the water solution. Gently mix it by shaking. Step two. After mixing, your solution … If chlorine solution is not prepared fresh daily, it can be stored at room temperature for up to 30 days in a capped, opaque plastic bottle with a 50% reduction in chlorine concentration after 30 days of storage (e.g., 1000 ppm chlorine [approximately a 1:50 dilution] at day 0 decreases to 500 ppm chlorine … Wearing the gloves, apply the solution to the hard surface, such as doorknobs or non-porous countertop. Step three. ** Step four Mixing the solution in this order will... Place the lid tightly on the container. Prepare a bleach solution by mixing: 5 tablespoons (1/3 rd cup) bleach per gallon of room temperature water or; 4 teaspoons bleach per quart of room temperature water; Bleach solutions will be effective for disinfection up to 24 hours. Soft (Porous) Surfaces To properly disinfect, the solution needs to be in contact with the surface for at least 5 minutes.