Size exclusion chromatography (SEC) is also referred to as gel chromatography, gel filtration, and gel permeation chromatography, is a technique in which particles or molecules in solution are separated for analysis via size exclusion.
Separation occurs when molecules of different sizes are included or excluded from the pores within the matrix.
However, even for molecules with the same size, some can have a low molecular weight with a loosely packed structure, while others can have a high molecular weight with a high-densely packed structure. The solution to be analyzed is introduced to the SEC column, which is packed with a stationary phase consisting of small porous beads.
Size exclusion chromatography (SEC or SEC-HPLC) for measurement of protein absolute molecular weight, structure, size and conformation Size Exclusion Chromatography (SEC or SEC-HPLC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel. As described in the beginning, size exclusion chromatography is a technique used to sort molecules by size. Unlike techniques such as
Size exclusion chromatography (SEC), also known as gel filtration, is the mildest of all the chromatography techniques.
SEC is generally used to separate biological molecules, to determine molecular weight distributions of proteins and peptides, as well as to separate a long list of water soluble polymers used in a wide range of industries.
SEC separates molecules by differences in size as they pass through a resin packed in a column. Size exclusion chromatography (SEC) separates molecules based on their size by filtration through a gel. In this method the largest molecules emerge from the chromatographic column first, because they are unable to penetrate the porous matrix of the support.
The aqueous separation techniques used were useful to purify the total saponin fraction, but failed to separate individual saponins due to the formation of mixed micelles. Dialysis, anion exchange, and size exclusion chromatography were used to produce a fraction consisting predominantly of saponin, also known as QuilA (Dalsgaard, 1974). Other articles where Size-exclusion chromatography is discussed: separation and purification: Exclusion and clathration: Size-exclusion chromatography (SEC) has proved effective for the separation and analysis of mixtures of polymers. Size exclusion chromatography (SEC) is a major mode of HPLC that employs porous particles in the column to separate molecules by virtue of their size in solution.
The gel consists of spherical beads containing pores of a specific size distribution.